Construct: shRNA TRCN0000155846

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015330.1-5429s1c1
DNA Barcode:
CCACTGATGTTCATGTGAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPECC1L (23384)
Gene Description:
sperm antigen with calponin homology and coiled-coil domains 1 like
Transcript:
RefSeq NM_015330.1 (NON-CURRENT)
Match location:
Position 5429 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155846)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGATGTTCATGTGAGAA
Hairpin Sequence:
5'-CCGG-CCACTGATGTTCATGTGAGAA-CTCGAG-TTCTCACATGAACATCAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGATGTTCATGTGAGAACTCGAGTTCTCACATGAACATCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGATGTTCATGTGAGAACTCGAGTTCTCACATGAACATCAGTGG-3'

Other clones with same target sequence:

(none)