Construct: shRNA TRCN0000155887

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_004749.2-792s1c1
DNA Barcode:
CACATTAGTGACCGTCATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TBRG4 (9238)
Gene Description:
transforming growth factor beta regulator 4
Transcript:
RefSeq NM_004749.2 (NON-CURRENT)
Match location:
Position 792 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155887)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACATTAGTGACCGTCATGAT
Hairpin Sequence:
5'-CCGG-CACATTAGTGACCGTCATGAT-CTCGAG-ATCATGACGGTCACTAATGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACATTAGTGACCGTCATGATCTCGAGATCATGACGGTCACTAATGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACATTAGTGACCGTCATGATCTCGAGATCATGACGGTCACTAATGTG-3'

Other clones with same target sequence:

(none)