Construct: shRNA TRCN0000155995

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005054.1-6481s1c1
DNA Barcode:
CCGTGCTACATCTTCACAGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RGPD5 (84220)
Gene Description:
RANBP2 like and GRIP domain containing 5
Transcript:
RefSeq NM_005054.1 (NON-CURRENT)
Match location:
Position 6481 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000155995)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGCTACATCTTCACAGTT
Hairpin Sequence:
5'-CCGG-CCGTGCTACATCTTCACAGTT-CTCGAG-AACTGTGAAGATGTAGCACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGCTACATCTTCACAGTTCTCGAGAACTGTGAAGATGTAGCACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGCTACATCTTCACAGTTCTCGAGAACTGTGAAGATGTAGCACGG-3'

Other clones with same target sequence:

(none)