Construct: shRNA TRCN0000156017

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183058.1-385s1c1
DNA Barcode:
CGGAAAGCTGAAGGAGAACAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LYZL2 (119180)
Gene Description:
lysozyme like 2
Transcript:
RefSeq NM_183058.1 (NON-CURRENT)
Match location:
Position 385 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156017)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGAAAGCTGAAGGAGAACAA
Hairpin Sequence:
5'-CCGG-CGGAAAGCTGAAGGAGAACAA-CTCGAG-TTGTTCTCCTTCAGCTTTCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGAAAGCTGAAGGAGAACAACTCGAGTTGTTCTCCTTCAGCTTTCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGAAAGCTGAAGGAGAACAACTCGAGTTGTTCTCCTTCAGCTTTCCG-3'

Other clones with same target sequence:

  1. TRCN0000049549