Construct: shRNA TRCN0000156025

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020195.1-624s1c1
DNA Barcode:
CACAGGTGTAGCTTACTACCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SDR39U1 (56948)
Gene Description:
short chain dehydrogenase/reductase family 39U member 1
Transcript:
RefSeq NM_020195.1 (NON-CURRENT)
Match location:
Position 624 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156025)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACAGGTGTAGCTTACTACCA
Hairpin Sequence:
5'-CCGG-CACAGGTGTAGCTTACTACCA-CTCGAG-TGGTAGTAAGCTACACCTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACAGGTGTAGCTTACTACCACTCGAGTGGTAGTAAGCTACACCTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACAGGTGTAGCTTACTACCACTCGAGTGGTAGTAAGCTACACCTGTG-3'

Other clones with same target sequence:

(none)