Construct: shRNA TRCN0000156105

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005883.2-8336s1c1
DNA Barcode:
CCAGATGAGGTGAAAGCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
APC2 (10297)
Gene Description:
APC regulator of WNT signaling pathway 2
Transcript:
RefSeq NM_005883.2 (CURRENT)
Match location:
Position 8336 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156105)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGATGAGGTGAAAGCTTAT
Hairpin Sequence:
5'-CCGG-CCAGATGAGGTGAAAGCTTAT-CTCGAG-ATAAGCTTTCACCTCATCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGATGAGGTGAAAGCTTATCTCGAGATAAGCTTTCACCTCATCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGATGAGGTGAAAGCTTATCTCGAGATAAGCTTTCACCTCATCTGG-3'

Other clones with same target sequence:

(none)