Construct: shRNA TRCN0000156109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014892.2-313s1c1
DNA Barcode:
CCAAATTACTAAGGCAGCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCAF8 (22828)
Gene Description:
SR-related CTD associated factor 8
Transcript:
RefSeq NM_014892.2 (NON-CURRENT)
Match location:
Position 313 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAATTACTAAGGCAGCCAT
Hairpin Sequence:
5'-CCGG-CCAAATTACTAAGGCAGCCAT-CTCGAG-ATGGCTGCCTTAGTAATTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAATTACTAAGGCAGCCATCTCGAGATGGCTGCCTTAGTAATTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAATTACTAAGGCAGCCATCTCGAGATGGCTGCCTTAGTAATTTGG-3'

Other clones with same target sequence:

(none)