Construct: shRNA TRCN0000156113

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015610.1-348s1c1
DNA Barcode:
CCCTAGCTGTTGGTAGTAAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WIPI2 (26100)
Gene Description:
WD repeat domain, phosphoinositide interacting 2
Transcript:
RefSeq NM_015610.1 (NON-CURRENT)
Match location:
Position 348 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156113)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTAGCTGTTGGTAGTAAGT
Hairpin Sequence:
5'-CCGG-CCCTAGCTGTTGGTAGTAAGT-CTCGAG-ACTTACTACCAACAGCTAGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTAGCTGTTGGTAGTAAGTCTCGAGACTTACTACCAACAGCTAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTAGCTGTTGGTAGTAAGTCTCGAGACTTACTACCAACAGCTAGGG-3'

Other clones with same target sequence:

(none)