Construct: shRNA TRCN0000156157

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153750.1-858s1c1
DNA Barcode:
CCTAGCACTGAAATGGAAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C21orf81 (114035)
Gene Description:
chromosome 21 open reading frame 81
Transcript:
RefSeq NM_153750.1 (NON-CURRENT)
Match location:
Position 858 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156157)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAGCACTGAAATGGAAGAT
Hairpin Sequence:
5'-CCGG-CCTAGCACTGAAATGGAAGAT-CTCGAG-ATCTTCCATTTCAGTGCTAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAGCACTGAAATGGAAGATCTCGAGATCTTCCATTTCAGTGCTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAGCACTGAAATGGAAGATCTCGAGATCTTCCATTTCAGTGCTAGG-3'

Other clones with same target sequence:

(none)