Construct: shRNA TRCN0000156212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024864.3-1433s1c1
DNA Barcode:
CAGAGGAAGCTGTTTCCTGTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MRM1 (79922)
Gene Description:
mitochondrial rRNA methyltransferase 1
Transcript:
RefSeq NM_024864.3 (NON-CURRENT)
Match location:
Position 1433 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGAGGAAGCTGTTTCCTGTT
Hairpin Sequence:
5'-CCGG-CAGAGGAAGCTGTTTCCTGTT-CTCGAG-AACAGGAAACAGCTTCCTCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGAGGAAGCTGTTTCCTGTTCTCGAGAACAGGAAACAGCTTCCTCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGAGGAAGCTGTTTCCTGTTCTCGAGAACAGGAAACAGCTTCCTCTG-3'

Other clones with same target sequence:

  1. TRCN0000285550