Construct: shRNA TRCN0000156297

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004065.2-2729s1c1
DNA Barcode:
CGCGAGAGAATGGATGATAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
AKAP2 (11217)
Gene Description:
A-kinase anchoring protein 2
Transcript:
RefSeq NM_001004065.2 (NON-CURRENT)
Match location:
Position 2729 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156297)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCGAGAGAATGGATGATAGT
Hairpin Sequence:
5'-CCGG-CGCGAGAGAATGGATGATAGT-CTCGAG-ACTATCATCCATTCTCTCGCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCGAGAGAATGGATGATAGTCTCGAGACTATCATCCATTCTCTCGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCGAGAGAATGGATGATAGTCTCGAGACTATCATCCATTCTCTCGCG-3'

Other clones with same target sequence:

(none)