Construct: shRNA TRCN0000156345

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182631.1-533s1c1
DNA Barcode:
CAGGAAGAGACGTGTTCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD18DP (348840)
Gene Description:
ankyrin repeat domain 18D, pseudogene
Transcript:
RefSeq NM_182631.1 (NON-CURRENT)
Match location:
Position 533 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156345)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGAAGAGACGTGTTCCATT
Hairpin Sequence:
5'-CCGG-CAGGAAGAGACGTGTTCCATT-CTCGAG-AATGGAACACGTCTCTTCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGAAGAGACGTGTTCCATTCTCGAGAATGGAACACGTCTCTTCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGAAGAGACGTGTTCCATTCTCGAGAATGGAACACGTCTCTTCCTG-3'

Other clones with same target sequence:

(none)