Construct: shRNA TRCN0000156447

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173833.2-671s1c1
DNA Barcode:
CAATGTGAACCGGCTGAATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCARA5 (286133)
Gene Description:
scavenger receptor class A member 5
Transcript:
RefSeq NM_173833.2 (NON-CURRENT)
Match location:
Position 671 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156447)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAATGTGAACCGGCTGAATGA
Hairpin Sequence:
5'-CCGG-CAATGTGAACCGGCTGAATGA-CTCGAG-TCATTCAGCCGGTTCACATTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAATGTGAACCGGCTGAATGACTCGAGTCATTCAGCCGGTTCACATTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAATGTGAACCGGCTGAATGACTCGAGTCATTCAGCCGGTTCACATTG-3'

Other clones with same target sequence:

(none)