Construct: shRNA TRCN0000156476

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178860.2-2278s1c1
DNA Barcode:
CGTAGCCACTTCAAGCTCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SEZ6 (124925)
Gene Description:
seizure related 6 homolog
Transcript:
RefSeq NM_178860.2 (NON-CURRENT)
Match location:
Position 2278 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156476)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAGCCACTTCAAGCTCTTT
Hairpin Sequence:
5'-CCGG-CGTAGCCACTTCAAGCTCTTT-CTCGAG-AAAGAGCTTGAAGTGGCTACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAGCCACTTCAAGCTCTTTCTCGAGAAAGAGCTTGAAGTGGCTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAGCCACTTCAAGCTCTTTCTCGAGAAAGAGCTTGAAGTGGCTACG-3'

Other clones with same target sequence:

(none)