Construct: shRNA TRCN0000156490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014801.2-1743s1c1
DNA Barcode:
CTGAGAGTCAAGATCCGTCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCNX2 (80003)
Gene Description:
pecanex 2
Transcript:
RefSeq NM_014801.2 (NON-CURRENT)
Match location:
Position 1743 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGAGAGTCAAGATCCGTCTT
Hairpin Sequence:
5'-CCGG-CTGAGAGTCAAGATCCGTCTT-CTCGAG-AAGACGGATCTTGACTCTCAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGAGAGTCAAGATCCGTCTTCTCGAGAAGACGGATCTTGACTCTCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGAGAGTCAAGATCCGTCTTCTCGAGAAGACGGATCTTGACTCTCAG-3'

Other clones with same target sequence:

  1. TRCN0000276039