Construct: shRNA TRCN0000156516

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030790.2-261s1c1
DNA Barcode:
GCTGCGGGAAAGAAATGACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ITFG1 (81533)
Gene Description:
integrin alpha FG-GAP repeat containing 1
Transcript:
RefSeq NM_030790.2 (NON-CURRENT)
Match location:
Position 261 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156516)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCGGGAAAGAAATGACTT
Hairpin Sequence:
5'-CCGG-GCTGCGGGAAAGAAATGACTT-CTCGAG-AAGTCATTTCTTTCCCGCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCGGGAAAGAAATGACTTCTCGAGAAGTCATTTCTTTCCCGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCGGGAAAGAAATGACTTCTCGAGAAGTCATTTCTTTCCCGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000343699