Construct: shRNA TRCN0000156711

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001318.2-302s1c1
DNA Barcode:
GCGATGACTATCACCTCCTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CSHL1 (1444)
Gene Description:
chorionic somatomammotropin hormone like 1
Transcript:
RefSeq NM_001318.2 (NON-CURRENT)
Match location:
Position 302 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156711)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGATGACTATCACCTCCTAA
Hairpin Sequence:
5'-CCGG-GCGATGACTATCACCTCCTAA-CTCGAG-TTAGGAGGTGATAGTCATCGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGATGACTATCACCTCCTAACTCGAGTTAGGAGGTGATAGTCATCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGATGACTATCACCTCCTAACTCGAGTTAGGAGGTGATAGTCATCGC-3'

Other clones with same target sequence:

  1. TRCN0000083813