Construct: shRNA TRCN0000156814

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153255.2-1171s1c1
DNA Barcode:
GCCCTCAAGTGTTTGGAATGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MCM9 (254394)
Gene Description:
minichromosome maintenance 9 homologous recombination repair factor
Transcript:
RefSeq NM_153255.2 (NON-CURRENT)
Match location:
Position 1171 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156814)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTCAAGTGTTTGGAATGT
Hairpin Sequence:
5'-CCGG-GCCCTCAAGTGTTTGGAATGT-CTCGAG-ACATTCCAAACACTTGAGGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTCAAGTGTTTGGAATGTCTCGAGACATTCCAAACACTTGAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTCAAGTGTTTGGAATGTCTCGAGACATTCCAAACACTTGAGGGC-3'

Other clones with same target sequence:

(none)