Construct: shRNA TRCN0000156939

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025078.3-742s1c1
DNA Barcode:
GAGCATCAAGATGGTGCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SLC66A2 (80148)
Gene Description:
solute carrier family 66 member 2
Transcript:
RefSeq NM_025078.3 (NON-CURRENT)
Match location:
Position 742 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000156939)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGCATCAAGATGGTGCTCAT
Hairpin Sequence:
5'-CCGG-GAGCATCAAGATGGTGCTCAT-CTCGAG-ATGAGCACCATCTTGATGCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGCATCAAGATGGTGCTCATCTCGAGATGAGCACCATCTTGATGCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGCATCAAGATGGTGCTCATCTCGAGATGAGCACCATCTTGATGCTC-3'

Other clones with same target sequence:

(none)