Construct: shRNA TRCN0000157013

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182538.3-563s1c1
DNA Barcode:
GCTGGCTGTCTTCTACATCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPNS3 (201305)
Gene Description:
sphingolipid transporter 3 (putative)
Transcript:
RefSeq NM_182538.3 (NON-CURRENT)
Match location:
Position 563 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157013)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGCTGTCTTCTACATCTT
Hairpin Sequence:
5'-CCGG-GCTGGCTGTCTTCTACATCTT-CTCGAG-AAGATGTAGAAGACAGCCAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGCTGTCTTCTACATCTTCTCGAGAAGATGTAGAAGACAGCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGCTGTCTTCTACATCTTCTCGAGAAGATGTAGAAGACAGCCAGC-3'

Other clones with same target sequence:

(none)