Construct: shRNA TRCN0000157049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001017418.1-484s1c1
DNA Barcode:
GAGACTGAGCAAAGGAACCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2B (6701)
Gene Description:
small proline rich protein 2B
Transcript:
RefSeq NM_001017418.1 (NON-CURRENT)
Match location:
Position 484 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGACTGAGCAAAGGAACCTT
Hairpin Sequence:
5'-CCGG-GAGACTGAGCAAAGGAACCTT-CTCGAG-AAGGTTCCTTTGCTCAGTCTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGACTGAGCAAAGGAACCTTCTCGAGAAGGTTCCTTTGCTCAGTCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGACTGAGCAAAGGAACCTTCTCGAGAAGGTTCCTTTGCTCAGTCTC-3'

Other clones with same target sequence:

(none)