Construct: shRNA TRCN0000157179

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001269.2-1004s1c1
DNA Barcode:
GTGAGATTCCAGGATGCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RCC1 (1104)
Gene Description:
regulator of chromosome condensation 1
Transcript:
RefSeq NM_001269.2 (NON-CURRENT)
Match location:
Position 1004 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157179)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTGAGATTCCAGGATGCCTTT
Hairpin Sequence:
5'-CCGG-GTGAGATTCCAGGATGCCTTT-CTCGAG-AAAGGCATCCTGGAATCTCAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTGAGATTCCAGGATGCCTTTCTCGAGAAAGGCATCCTGGAATCTCACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTGAGATTCCAGGATGCCTTTCTCGAGAAAGGCATCCTGGAATCTCAC-3'

Other clones with same target sequence:

(none)