Construct: shRNA TRCN0000157202

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152531.3-1931s1c1
DNA Barcode:
GCTTGTATCTCCCAGCTACAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
XXYLT1 (152002)
Gene Description:
xyloside xylosyltransferase 1
Transcript:
RefSeq NM_152531.3 (NON-CURRENT)
Match location:
Position 1931 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157202)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTTGTATCTCCCAGCTACAT
Hairpin Sequence:
5'-CCGG-GCTTGTATCTCCCAGCTACAT-CTCGAG-ATGTAGCTGGGAGATACAAGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTTGTATCTCCCAGCTACATCTCGAGATGTAGCTGGGAGATACAAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTTGTATCTCCCAGCTACATCTCGAGATGTAGCTGGGAGATACAAGC-3'

Other clones with same target sequence:

(none)