Construct: shRNA TRCN0000157275

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001008777.1-1743s1c1
DNA Barcode:
GCCTCAAGAATGTGCTGAGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FBXO47 (494188)
Gene Description:
F-box protein 47
Transcript:
RefSeq NM_001008777.1 (NON-CURRENT)
Match location:
Position 1743 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157275)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTCAAGAATGTGCTGAGAT
Hairpin Sequence:
5'-CCGG-GCCTCAAGAATGTGCTGAGAT-CTCGAG-ATCTCAGCACATTCTTGAGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTCAAGAATGTGCTGAGATCTCGAGATCTCAGCACATTCTTGAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTCAAGAATGTGCTGAGATCTCGAGATCTCAGCACATTCTTGAGGC-3'

Other clones with same target sequence:

(none)