Construct: shRNA TRCN0000157281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001090.1-391s1c1
DNA Barcode:
GCGTCTTAAGAAGCTCTCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ABCF1 (23)
Gene Description:
ATP binding cassette subfamily F member 1
Transcript:
RefSeq NM_001090.1 (NON-CURRENT)
Match location:
Position 391 (CDS)
CRISPR Mechanism
CRISPR

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTCTTAAGAAGCTCTCAGT
Hairpin Sequence:
5'-CCGG-GCGTCTTAAGAAGCTCTCAGT-CTCGAG-ACTGAGAGCTTCTTAAGACGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTCTTAAGAAGCTCTCAGTCTCGAGACTGAGAGCTTCTTAAGACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTCTTAAGAAGCTCTCAGTCTCGAGACTGAGAGCTTCTTAAGACGC-3'

Other clones with same target sequence:

(none)