Construct: shRNA TRCN0000157369

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033083.5-1027s1c1
DNA Barcode:
CTTCGGGAAGTGATGACGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
EAF1 (85403)
Gene Description:
ELL associated factor 1
Transcript:
RefSeq NM_033083.5 (NON-CURRENT)
Match location:
Position 1027 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157369)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCGGGAAGTGATGACGATA
Hairpin Sequence:
5'-CCGG-CTTCGGGAAGTGATGACGATA-CTCGAG-TATCGTCATCACTTCCCGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCGGGAAGTGATGACGATACTCGAGTATCGTCATCACTTCCCGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCGGGAAGTGATGACGATACTCGAGTATCGTCATCACTTCCCGAAG-3'

Other clones with same target sequence:

(none)