Construct: shRNA TRCN0000157378

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175723.1-399s1c1
DNA Barcode:
CCAGCAGAGGAAGGAAATGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SSX5 (6758)
Gene Description:
SSX family member 5
Transcript:
RefSeq NM_175723.1 (CURRENT)
Match location:
Position 399 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157378)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGCAGAGGAAGGAAATGAT
Hairpin Sequence:
5'-CCGG-CCAGCAGAGGAAGGAAATGAT-CTCGAG-ATCATTTCCTTCCTCTGCTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGCAGAGGAAGGAAATGATCTCGAGATCATTTCCTTCCTCTGCTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGCAGAGGAAGGAAATGATCTCGAGATCATTTCCTTCCTCTGCTGG-3'

Other clones with same target sequence:

  1. TRCN0000115839