Construct: shRNA TRCN0000157478

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002643.2-526s1c1
DNA Barcode:
GCATGGCTAAGAGTGTTCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIGF (5281)
Gene Description:
phosphatidylinositol glycan anchor biosynthesis class F
Transcript:
RefSeq NM_002643.2 (NON-CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157478)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCATGGCTAAGAGTGTTCAGT
Hairpin Sequence:
5'-CCGG-GCATGGCTAAGAGTGTTCAGT-CTCGAG-ACTGAACACTCTTAGCCATGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCATGGCTAAGAGTGTTCAGTCTCGAGACTGAACACTCTTAGCCATGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCATGGCTAAGAGTGTTCAGTCTCGAGACTGAACACTCTTAGCCATGC-3'

Other clones with same target sequence:

  1. TRCN0000338616