Construct: shRNA TRCN0000157534

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152557.3-1424s1c1
DNA Barcode:
GCCATTCAACGAACCCTGTAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF746 (155061)
Gene Description:
zinc finger protein 746
Transcript:
RefSeq NM_152557.3 (NON-CURRENT)
Match location:
Position 1424 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157534)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCATTCAACGAACCCTGTAA
Hairpin Sequence:
5'-CCGG-GCCATTCAACGAACCCTGTAA-CTCGAG-TTACAGGGTTCGTTGAATGGC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCATTCAACGAACCCTGTAACTCGAGTTACAGGGTTCGTTGAATGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCATTCAACGAACCCTGTAACTCGAGTTACAGGGTTCGTTGAATGGC-3'

Other clones with same target sequence:

(none)