Construct: shRNA TRCN0000157541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001188.2-1883s1c1
DNA Barcode:
GACCTTCTCATTTGGGCCATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TRPM2 (7226)
Gene Description:
transient receptor potential cation channel subfamily M member 2
Transcript:
RefSeq NM_001001188.2 (NON-CURRENT)
Match location:
Position 1883 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GACCTTCTCATTTGGGCCATT
Hairpin Sequence:
5'-CCGG-GACCTTCTCATTTGGGCCATT-CTCGAG-AATGGCCCAAATGAGAAGGTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGACCTTCTCATTTGGGCCATTCTCGAGAATGGCCCAAATGAGAAGGTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGACCTTCTCATTTGGGCCATTCTCGAGAATGGCCCAAATGAGAAGGTC-3'

Other clones with same target sequence:

(none)