Construct: shRNA TRCN0000157562

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001029999.1-500s1c1
DNA Barcode:
GAACTGCTCTACATTTGGCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD20A5P (440482)
Gene Description:
ankyrin repeat domain 20 family member A5, pseudogene
Transcript:
RefSeq NM_001029999.1 (NON-CURRENT)
Match location:
Position 500 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157562)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAACTGCTCTACATTTGGCCT
Hairpin Sequence:
5'-CCGG-GAACTGCTCTACATTTGGCCT-CTCGAG-AGGCCAAATGTAGAGCAGTTC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAACTGCTCTACATTTGGCCTCTCGAGAGGCCAAATGTAGAGCAGTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAACTGCTCTACATTTGGCCTCTCGAGAGGCCAAATGTAGAGCAGTTC-3'

Other clones with same target sequence:

  1. TRCN0000140773