Construct: shRNA TRCN0000157588

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025216.2-577s1c1
DNA Barcode:
GTCAGCACCCAATGACATTCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
WNT10A (80326)
Gene Description:
Wnt family member 10A
Transcript:
RefSeq NM_025216.2 (NON-CURRENT)
Match location:
Position 577 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157588)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCAGCACCCAATGACATTCT
Hairpin Sequence:
5'-CCGG-GTCAGCACCCAATGACATTCT-CTCGAG-AGAATGTCATTGGGTGCTGAC-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCAGCACCCAATGACATTCTCTCGAGAGAATGTCATTGGGTGCTGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCAGCACCCAATGACATTCTCTCGAGAGAATGTCATTGGGTGCTGAC-3'

Other clones with same target sequence:

(none)