Construct: shRNA TRCN0000157883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006945.3-498s1c1
DNA Barcode:
CAGGGAGCTTCTTTCTCCTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SPRR2D (6703)
Gene Description:
small proline rich protein 2D
Transcript:
RefSeq NM_006945.3 (NON-CURRENT)
Match location:
Position 498 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGGAGCTTCTTTCTCCTTA
Hairpin Sequence:
5'-CCGG-CAGGGAGCTTCTTTCTCCTTA-CTCGAG-TAAGGAGAAAGAAGCTCCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGGAGCTTCTTTCTCCTTACTCGAGTAAGGAGAAAGAAGCTCCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGGAGCTTCTTTCTCCTTACTCGAGTAAGGAGAAAGAAGCTCCCTG-3'

Other clones with same target sequence:

(none)