Construct: shRNA TRCN0000157892

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021260.1-4134s1c1
DNA Barcode:
CCCGTTGTGCTGAAATCCTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZFYVE1 (53349)
Gene Description:
zinc finger FYVE-type containing 1
Transcript:
RefSeq NM_021260.1 (NON-CURRENT)
Match location:
Position 4134 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157892)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCGTTGTGCTGAAATCCTAT
Hairpin Sequence:
5'-CCGG-CCCGTTGTGCTGAAATCCTAT-CTCGAG-ATAGGATTTCAGCACAACGGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCGTTGTGCTGAAATCCTATCTCGAGATAGGATTTCAGCACAACGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCGTTGTGCTGAAATCCTATCTCGAGATAGGATTTCAGCACAACGGG-3'

Other clones with same target sequence:

(none)