Construct: shRNA TRCN0000157899

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153750.1-754s1c1
DNA Barcode:
CCAAGAAAGTGTCAGAGCCTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C21orf81 (114035)
Gene Description:
chromosome 21 open reading frame 81
Transcript:
RefSeq NM_153750.1 (NON-CURRENT)
Match location:
Position 754 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157899)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGAAAGTGTCAGAGCCTT
Hairpin Sequence:
5'-CCGG-CCAAGAAAGTGTCAGAGCCTT-CTCGAG-AAGGCTCTGACACTTTCTTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGAAAGTGTCAGAGCCTTCTCGAGAAGGCTCTGACACTTTCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGAAAGTGTCAGAGCCTTCTCGAGAAGGCTCTGACACTTTCTTGG-3'

Other clones with same target sequence:

(none)