Construct: shRNA TRCN0000157984

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003928.1-981s1c1
DNA Barcode:
CAGCCAAATCGAGTCTCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RTL8C (8933)
Gene Description:
retrotransposon Gag like 8C
Transcript:
RefSeq NM_003928.1 (NON-CURRENT)
Match location:
Position 981 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000157984)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGCCAAATCGAGTCTCTCAT
Hairpin Sequence:
5'-CCGG-CAGCCAAATCGAGTCTCTCAT-CTCGAG-ATGAGAGACTCGATTTGGCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGCCAAATCGAGTCTCTCATCTCGAGATGAGAGACTCGATTTGGCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGCCAAATCGAGTCTCTCATCTCGAGATGAGAGACTCGATTTGGCTG-3'

Other clones with same target sequence:

  1. TRCN0000322939