Construct: shRNA TRCN0000158028

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024611.3-3043s1c1
DNA Barcode:
CCACAGGAATCCAGTTTCCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ICE2 (79664)
Gene Description:
interactor of little elongation complex ELL subunit 2
Transcript:
RefSeq NM_024611.3 (NON-CURRENT)
Match location:
Position 3043 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158028)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACAGGAATCCAGTTTCCAT
Hairpin Sequence:
5'-CCGG-CCACAGGAATCCAGTTTCCAT-CTCGAG-ATGGAAACTGGATTCCTGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACAGGAATCCAGTTTCCATCTCGAGATGGAAACTGGATTCCTGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACAGGAATCCAGTTTCCATCTCGAGATGGAAACTGGATTCCTGTGG-3'

Other clones with same target sequence:

(none)