Construct: shRNA TRCN0000158063

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_022104.3-851s1c1
DNA Barcode:
CATCCAGACCAATGCTGTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PCIF1 (63935)
Gene Description:
PDX1 C-terminal inhibiting factor 1
Transcript:
RefSeq NM_022104.3 (NON-CURRENT)
Match location:
Position 851 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158063)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCCAGACCAATGCTGTCAT
Hairpin Sequence:
5'-CCGG-CATCCAGACCAATGCTGTCAT-CTCGAG-ATGACAGCATTGGTCTGGATG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCCAGACCAATGCTGTCATCTCGAGATGACAGCATTGGTCTGGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCCAGACCAATGCTGTCATCTCGAGATGACAGCATTGGTCTGGATG-3'

Other clones with same target sequence:

  1. TRCN0000285500