Construct: shRNA TRCN0000158081

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012289.3-766s1c1
DNA Barcode:
CGGGAGTACATCTACATGCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KEAP1 (9817)
Gene Description:
kelch like ECH associated protein 1
Transcript:
RefSeq NM_012289.3 (NON-CURRENT)
Match location:
Position 766 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158081)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGGAGTACATCTACATGCAT
Hairpin Sequence:
5'-CCGG-CGGGAGTACATCTACATGCAT-CTCGAG-ATGCATGTAGATGTACTCCCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGGAGTACATCTACATGCATCTCGAGATGCATGTAGATGTACTCCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGGAGTACATCTACATGCATCTCGAGATGCATGTAGATGTACTCCCG-3'

Other clones with same target sequence:

  1. TRCN0000280616