Construct: shRNA TRCN0000158083

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030627.1-960s1c1
DNA Barcode:
CCAGTGTTGACAGGGTTTGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CPEB4 (80315)
Gene Description:
cytoplasmic polyadenylation element binding protein 4
Transcript:
RefSeq NM_030627.1 (NON-CURRENT)
Match location:
Position 960 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158083)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAGTGTTGACAGGGTTTGAT
Hairpin Sequence:
5'-CCGG-CCAGTGTTGACAGGGTTTGAT-CTCGAG-ATCAAACCCTGTCAACACTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAGTGTTGACAGGGTTTGATCTCGAGATCAAACCCTGTCAACACTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAGTGTTGACAGGGTTTGATCTCGAGATCAAACCCTGTCAACACTGG-3'

Other clones with same target sequence:

(none)