Construct: shRNA TRCN0000158109

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_003027.2-1628s1c1
DNA Barcode:
CGGTGTTCTGTGACATCCTTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SH3GL3 (6457)
Gene Description:
SH3 domain containing GRB2 like 3, endophilin A3
Transcript:
RefSeq NM_003027.2 (NON-CURRENT)
Match location:
Position 1628 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158109)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTGTTCTGTGACATCCTTT
Hairpin Sequence:
5'-CCGG-CGGTGTTCTGTGACATCCTTT-CTCGAG-AAAGGATGTCACAGAACACCG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTGTTCTGTGACATCCTTTCTCGAGAAAGGATGTCACAGAACACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTGTTCTGTGACATCCTTTCTCGAGAAAGGATGTCACAGAACACCG-3'

Other clones with same target sequence:

(none)