Construct: shRNA TRCN0000158124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018151.1-5590s1c1
DNA Barcode:
CCGTGTTTAGGAGACTCGAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RIF1 (55183)
Gene Description:
replication timing regulatory factor 1
Transcript:
RefSeq NM_018151.1 (NON-CURRENT)
Match location:
Position 5590 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGTGTTTAGGAGACTCGAAA
Hairpin Sequence:
5'-CCGG-CCGTGTTTAGGAGACTCGAAA-CTCGAG-TTTCGAGTCTCCTAAACACGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGTGTTTAGGAGACTCGAAACTCGAGTTTCGAGTCTCCTAAACACGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGTGTTTAGGAGACTCGAAACTCGAGTTTCGAGTCTCCTAAACACGG-3'

Other clones with same target sequence:

(none)