Construct: shRNA TRCN0000158169

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144660.1-1141s1c1
DNA Barcode:
CGTGACTGAGAAGCATTGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SAMD8 (142891)
Gene Description:
sterile alpha motif domain containing 8
Transcript:
RefSeq NM_144660.1 (NON-CURRENT)
Match location:
Position 1141 (3UTR)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158169)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTGACTGAGAAGCATTGGAA
Hairpin Sequence:
5'-CCGG-CGTGACTGAGAAGCATTGGAA-CTCGAG-TTCCAATGCTTCTCAGTCACG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTGACTGAGAAGCATTGGAACTCGAGTTCCAATGCTTCTCAGTCACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTGACTGAGAAGCATTGGAACTCGAGTTCCAATGCTTCTCAGTCACG-3'

Other clones with same target sequence:

(none)