Construct: shRNA TRCN0000158170

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152557.3-875s1c1
DNA Barcode:
CTCTGGTGTCCATTCCAACTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF746 (155061)
Gene Description:
zinc finger protein 746
Transcript:
RefSeq NM_152557.3 (NON-CURRENT)
Match location:
Position 875 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158170)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCTGGTGTCCATTCCAACTT
Hairpin Sequence:
5'-CCGG-CTCTGGTGTCCATTCCAACTT-CTCGAG-AAGTTGGAATGGACACCAGAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCTGGTGTCCATTCCAACTTCTCGAGAAGTTGGAATGGACACCAGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCTGGTGTCCATTCCAACTTCTCGAGAAGTTGGAATGGACACCAGAG-3'

Other clones with same target sequence:

(none)