Construct: shRNA TRCN0000158207

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182795.1-366s1c1
DNA Barcode:
CAGGCTGTTGCTTCATACGAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NPM2 (10361)
Gene Description:
nucleophosmin/nucleoplasmin 2
Transcript:
RefSeq NM_182795.1 (CURRENT)
Match location:
Position 366 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158207)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAGGCTGTTGCTTCATACGAT
Hairpin Sequence:
5'-CCGG-CAGGCTGTTGCTTCATACGAT-CTCGAG-ATCGTATGAAGCAACAGCCTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAGGCTGTTGCTTCATACGATCTCGAGATCGTATGAAGCAACAGCCTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAGGCTGTTGCTTCATACGATCTCGAGATCGTATGAAGCAACAGCCTG-3'

Other clones with same target sequence:

(none)