Construct: shRNA TRCN0000158209

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_000132.2-1876s1c1
DNA Barcode:
CCTCTCCTCATCTGCTACAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
F8 (2157)
Gene Description:
coagulation factor VIII
Transcript:
RefSeq NM_000132.2 (NON-CURRENT)
Match location:
Position 1876 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158209)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTCCTCATCTGCTACAAA
Hairpin Sequence:
5'-CCGG-CCTCTCCTCATCTGCTACAAA-CTCGAG-TTTGTAGCAGATGAGGAGAGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTCCTCATCTGCTACAAACTCGAGTTTGTAGCAGATGAGGAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTCCTCATCTGCTACAAACTCGAGTTTGTAGCAGATGAGGAGAGG-3'

Other clones with same target sequence:

(none)