Construct: shRNA TRCN0000158210

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013618.1-526s1c1
DNA Barcode:
CACACTGGTGTGTCTCATGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IGLL3P (91353)
Gene Description:
immunoglobulin lambda like polypeptide 3, pseudogene
Transcript:
RefSeq NM_001013618.1 (NON-CURRENT)
Match location:
Position 526 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158210)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACACTGGTGTGTCTCATGAA
Hairpin Sequence:
5'-CCGG-CACACTGGTGTGTCTCATGAA-CTCGAG-TTCATGAGACACACCAGTGTG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACACTGGTGTGTCTCATGAACTCGAGTTCATGAGACACACCAGTGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACACTGGTGTGTCTCATGAACTCGAGTTCATGAGACACACCAGTGTG-3'

Other clones with same target sequence:

(none)