Construct: shRNA TRCN0000158279

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018682.2-3572s1c1
DNA Barcode:
CTTCCGAAGATGGGCTTGTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KMT2E (55904)
Gene Description:
lysine methyltransferase 2E
Transcript:
RefSeq NM_018682.2 (NON-CURRENT)
Match location:
Position 3572 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158279)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCCGAAGATGGGCTTGTAT
Hairpin Sequence:
5'-CCGG-CTTCCGAAGATGGGCTTGTAT-CTCGAG-ATACAAGCCCATCTTCGGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCCGAAGATGGGCTTGTATCTCGAGATACAAGCCCATCTTCGGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCCGAAGATGGGCTTGTATCTCGAGATACAAGCCCATCTTCGGAAG-3'

Other clones with same target sequence:

(none)