Construct: shRNA TRCN0000158312

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_020873.3-2377s1c1
DNA Barcode:
CCACTCATTAACCTCTGGGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
LRRN1 (57633)
Gene Description:
leucine rich repeat neuronal 1
Transcript:
RefSeq NM_020873.3 (NON-CURRENT)
Match location:
Position 2377 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158312)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTCATTAACCTCTGGGAA
Hairpin Sequence:
5'-CCGG-CCACTCATTAACCTCTGGGAA-CTCGAG-TTCCCAGAGGTTAATGAGTGG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTCATTAACCTCTGGGAACTCGAGTTCCCAGAGGTTAATGAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTCATTAACCTCTGGGAACTCGAGTTCCCAGAGGTTAATGAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000108482