Construct: shRNA TRCN0000158320

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032296.2-2433s1c1
DNA Barcode:
CTTCAAGACGTGTTCTCCTGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FLYWCH1 (84256)
Gene Description:
FLYWCH-type zinc finger 1
Transcript:
RefSeq NM_032296.2 (CURRENT)
Match location:
Position 2433 (CDS)

Vector Information:

Vector Backbone:
pLKO.1
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000158320)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTCAAGACGTGTTCTCCTGA
Hairpin Sequence:
5'-CCGG-CTTCAAGACGTGTTCTCCTGA-CTCGAG-TCAGGAGAACACGTCTTGAAG-TTTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTCAAGACGTGTTCTCCTGACTCGAGTCAGGAGAACACGTCTTGAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTCAAGACGTGTTCTCCTGACTCGAGTCAGGAGAACACGTCTTGAAG-3'

Other clones with same target sequence:

(none)